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SEMESTER: 4th FULL COURSE DETAILS

 SEMESTER: 4th      FULL COURSE DETAILS



 

Semester IV

Course No.

Course Title

Cr. Hours

BT- 221

Metabolism- I

3

BT- 222

Nucleic acid

3

BT- 223

Animal Physiology

3

BT- 224

Plant Physiology

3

BT-225

English-III (Comm Skill)

3

BT-226

General-III Basics of Financial Management)

3

Fourth Semester Total

18

 


SEMESTER IV

METABOLISM-I

BT-221                                                                                                                   (3 Credit hours)                                        

 

Course Contents:

            Introduction to metabolism; carbohydrate metabolism, sources of glucose to the organism (exogenous and endogenous) digestion and absorption of carbohydrates, utilization and storage of glucose, anaerobic oxidation of glucose, aerobic oxidation of glucose (TCA cycle), alternate pathways of carbohydrate metabolism (HMP shunt), gluconeogenesis, glycogen metabolism. Electron transport chain.

Lipid metabolism; digestion, absorption and transport of lipids, utilization and storage of lipids, fatty acids, oxidation, outline of biosynthesis of fatty acids, glycosides phospholipids, glycolipids, sterols and steroids. Regulation of metabolism. Integration, controls and disorders of metabolic pathways related to carbohydrates and lipids.

 

Practicals:

1.      Determination of Acid value of Fats.

2.      Determination of Lipid Composition of Wheat grain.

3.      Variation of Serum Alkaline Phosphatase activity with enzyme concentration.

4.      Effect of Substrate Concentration and inhibitors on ox heart Lactate Dehydrogenase.

5.      Effect of temperature on the activity of a- amylase.  

6.      Yeast isocitrate Dehydrogenase, Allosteric enzyme.

7.      lsolation of Muramidase from egg white. Fraction collector, (UV, VIS).

8.      Separation of isoenzyme of lactate Dehydrogenase by electrophoresis on polyacrylamide gel   (Equipment PAG).

9.      Some properties of Lactate Dehydrogenase Isoenzymes.

10.  Effect of Lipid composition on the permeability of a lipid monolayer.

 

Recommended Books:         

1.      Stryer L, Berg J, Tymoczky J (2002) 5th edition, “Biochemistry” W.HFreeman and Cvompany.

2.      David L. Nelson P, Michael M. Lehninger P (2004) 4th edition, “Principle of Biochemistry” W.H. Freeman & Company.

3.      Donald V (2004) 3rd edition, “Biochemistry” Wiley Text Books Corp.

4.      Christopher K. Mathews P, Kensal E, Kevin G (1999) 3rd edition, “Biochemistry” Pearson Education Company

5.      Murray R, Granuea D, Mayer P, Rodwell V (2003) 26th edition, “Harper’s illustrated Biochemistry” Mc Graw Hill Inc.

 

NUCLEIC ACID

BT-222                                                                                                                   (3 Credit hours)                                        

 

Course Contents:

Human genome organization; human chromosomes, gene structure, mutations, nuclear and mitochondria genome. Human multigene families; repetitive DNA sequences, pseudogenes. Expression of human genome; expression control at DNA, RNA, and protein levels, expression control at nucleosome level, genetic imprinting, X chromosome inactivation, immunoglobulin gene organization. Cloning of human genes, cDNA cloning, positional cloning of genes. Mutation detection. Human gene structure and function.

 

Practicals:

            Determination of UV absorption spectra of Nucleic Acids and quantification of DNA and RNA. Preparation of Plasmid DNA. RNA extraction and purification. Southern blot and hybridization. RNA electrophoresis. Amplification of human gene on PCR. Separation of alleles on vertical non-denaturing gel.

Recommended Books:

1.      Glick B, Pasternack J (1998) 2nd edition, “Molecular Biotechnology” ASM Pres   Washington DC.

2.  Strachan T, Andrew P (2004) 3rd edition, “Human   Molecular    Genetics” Willey and Sons.

3.   Stryer L, Berg j, Tymoczky J (2002) 5th edition, “Biochemistry”W.H Freeman      and Company.

 

ANIMAL PHYSIOLOGY

BT-223                                                                                                                   (3 Credit hours)                                        

 

Course Contents:

Introduction. Homeostasis. Biomembranes. Membrane potential. Nervous system; sensory physiology - chemoreception, mechanoreceptor photoreception. Muscle physiology; introduction, molecular structure, muscle contraction, skeletal muscle mechanics, - skeletal muscle movement, smooth and cardiac muscle, invertebrate muscles. Cardiac physiology; introduction to cardiac cycle, vertebrate & invertebrate. Cardiovascular system; introduction, solute exchange, blood pressure. Respiratory system; introduction, gas exchange & transport, control. Respiratory system of amphibians, reptiles and birds. Respiratory system; the vertebrate gill and water breathing. Other methods of gas exchange. Urinary system; vertebrate kidney, osmoregulation, extra renal osmoregulatory organs.

Fluid and acid-base balance. Digestive system - specialized digestion.

 

Practicals:

Dissection of frog and study of digestive, reproduction, arterial, venous and respiratory system. Blood cells. Dissection of pigeon and study of digestive, excretory, respiratory and reproduction system. Dissection of mouse and study of digestive, reproductive, excretory system and blood cells (White blood cells, red blood cells). Study of Nervous tissue (brain) of Mammals.

Recommended Books:

1.      Richard W, Gordon A, Margaret A (2004) 1st edition, “Animal Physiology” Mc Graw Hill Inc.

2.      Guyton (2000) 9th edition, “Text book of medical physiology” Mc Graw Hill Inc.

 

PLANT PHYSIOLOGY

BT-224                                                                                                                   (3 Credit hours)                                            

 

Course contents:

Plant structure; introduction to tissue and tissue system in primary plant body. Water relations; plant-soil-atmosphere continuum. (an overview). Physico-chemical properties of water. Water in the soil and its potentials. Water absorption. Plant mineral nutrition; importance of minerals, their absorption and role in plant development. Ascent of sap; path and theories. Mechanism of phloem transport. Leaves and atmosphere; gaseous exchange, mechanism and factors affecting stomatal movement. Photosynthesis; concept, an overview of the mechanism. Pigments and photosynthetic activity, photosystem I and II, photophosphorylation and its mechanism, dark reactions, (CO2 reduction). C4 and CAM pathways. Respiration; concept, respiratory substrates and quotient, aerobic and anaerobic respiration, Glycolysis, electron transport, ATP formation and oxidative photophosphorylation, factors affecting respiration. Fermentation, control and factors affecting respiration. Plant growth and development; phytohormones and role in biological processes, phytohormones-kinds, structure and mechanism of action, physiological effects of auxins, gibberellins,  cytokinins, abscisic acid and ethylene, control of flowering metabolic aspects of senescence, role of hormones in seed germination, aging and death. Photoperiodism and  mechanism. Vernalization.

 


 

Practicals:

Identification of C3 and C4 leaves. Identification of stomatal types. Study of special epidermal cells. Study of transverse section of root. Study of transverse section of stem. Determination of water potential by Chardakov’s method. Effect of light on stomatal movements. Extraction of chlorophyll from leaves and separation of pigments. Measurement of relative growth rate (RGR). Measurement of specific leaf area and plant activity. To observe that oxygen is released during photosynthesis.

Recommended books

1.      Kochar P, Krishna N (1994) 2nd edition, “A text book of plant physiology” Mc Graw Hill Book Company Inc.

2.      Giese C (1999) 2nd edition, “Cell Physiology” Mc Graw Hill Book Company Inc.

3.      Jain K (2000) 3rd edition, “Fundamentals of plant physiology” Mc Graw Hill Book   Company Inc.

4.      Ihsan Ilahi “Plant Physiology: Biophysical process in Plants” University Grant Commission, Islamabad.

5.      Ihsan Ilahi “Plant Physiology: Biochemical process in Plants” University grant commission, Islamabad.

ENGLISH-III (COMMUNICATION SKILLS)

BT-225                                                     (3 Credit hours)

 

 

GENERAL-III (BASIC OF FINANCIAL MANAGEMENT )

BT-226                                                    (3 Credit hours)

Chapter 1st:  introduction:      

i.                    Introduction to Management:

Management, Process of Management, types of Management

ii.                  Introduction to Financial Management

Financial Management, types of Financial Management, (Personal finance, corporate finance).

iii.                Financial Techniques, Concepts

Chapter 2nd:  Working Capital Management

i.                    Cash budget:

Need of Cash, Cash Importance, Cash Book Maintenance, Cash Flow Statement.

ii.                  Credit 

Credit policy, Advantages / disadvantages of credit trade Stock

iii.                stock control.

Chapter 3rd:  Management of fixed assets

i.                 Depreciation

ii.               Insurance

Chapter 4th:     Mathematical Management.

i.                    Calculation of Interest Rate simple interest rate, compound interest rate, present value techniques.

ii.                   The methods of appraising an investment proposal.

Pay back. Average Rate of Return, Internal Rate of Return, Present Value, Net Present Value

 

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